Target-fueled DNA walker for highly selective miRNA detection.

Target-fueled DNA walker for highly selective miRNA detection† †Electronic supplementary information (ESI) available: DNA strand structure and sequences, assembly of DNA strands as noted in the text. See DOI: 10.1039/c5sc02784e Click here for additional data file.

Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres.

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA.

Highly selective detection of microRNA based on distance-dependent electrochemiluminescence resonance energy transfer between CdTe nanocrystals and Au nanoclusters.

A distance-dependent electrochemiluminescence resonance energy transfer (ERET) system based on CdTe nanocrystals and Au nanoclusters (Au NCs) was designed with the aid of ligase for highly selective detection of microRNA (miRNA). First, Au NCs functionalized hairpin DNA was synthesized via Au-S chemistry, and characterized with transmission electron microscopy and dynamic light scattering. The ...

Highly Sensitive Microrna Detection Using Gold-nano-particles on Power-free Microfluidic Chip: towards Point-of-care Early-stage Cancer Diagnosis

We present a new method of rapid sequence-selective microRNA (miRNA) detection with small amount of sample using power-free PDMS microfluidic device and gold-nanoparticles (GNPs). Target miRNA hybridized to probe DNA was labeled by DNA-coated GNPs, which also act as signal amplifiers. This method allows us to detect miRNA of certain sequence in 30 min with 0.5 μL sample volume. This method migh...

A simple and highly sensitive fluorescence assay for microRNAs.

Herein, we have reported a simple and highly sensitive fluorescence assay for the detection of microRNAs (miRNAs). The assay uses a duplex-specific nuclease (DSN) to amplify the fluorescence signal and magnetic beads (MBs) to completely remove the unreacted DNA detection probes. Briefly, fluorescein-capped DNA detection probes were first conjugated to the MBs. The use of the MBs produced a very...